qdoR [2018-02-12T11:42:35.000Z]

transcriptional regulator (TetR family), control of qdoI-yxaH and lmrA-lmrB

locus: BSU39990

pI: 6.11

mw: 20.87 kDa

protein length: 191 aa

gene length: 573 bp

function: control of quercetin utilization

product: transcriptional regulator

essential: no

synonyms: yxaF

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.10|Resistance against other toxic compounds (nitric oxide, phenolic acids, flavonoids, oxalate)]

Gene

Coordinates: 4,107,352 → 4,107,927

The protein

Protein family

• [SW|TetR family]

Paralogous protein(s)

• [protein|52D560AA02F0849CB24460A496021560063B2E12|LmrA]

Effectors of protein activity

• flavonoids such as quercetin act as molecular inducers and result in release from DNA targets [Pubmed|17483215,21737930]

Structure

• [PDB|1SGM]

Expression and Regulation

Operons

• genes: [gene|47387014B5E89BF218BA94D8C2A570479B1EFD4E|qdoR]
  description: [Pubmed|10746760]

  regulatory mechanism

  • [protein|52D560AA02F0849CB24460A496021560063B2E12|LmrA]: repression, [Pubmed|15317768], in [regulon|52D560AA02F0849CB24460A496021560063B2E12|LmrA regulon]
  • [protein|47387014B5E89BF218BA94D8C2A570479B1EFD4E|QdoR]: repression, [Pubmed|17483215], in [regulon|47387014B5E89BF218BA94D8C2A570479B1EFD4E|QdoR regulon]

  regulation

  • induced by flavonoids such as quercetin ([protein|search|QdoR], [protein|search|LmrA]) [Pubmed|17483215]

Biological materials

Mutant

• MGNA-B683 (yxaF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1682 NBRP B. subtilis, Japan]
• BKE39990 (Δ[gene|47387014B5E89BF218BA94D8C2A570479B1EFD4E|qdoR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAAAACATTCCCTCCTA, downstream forward: _UP4_TAGCATTTTGTTATCCTTTC
• BKK39990 (Δ[gene|47387014B5E89BF218BA94D8C2A570479B1EFD4E|qdoR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAAAACATTCCCTCCTA, downstream forward: _UP4_TAGCATTTTGTTATCCTTTC

Labs working on this gene/protein

• [SW|Yasutaro Fujita], University of Fukuyama, Japan

References

Reviews

• 24006471,25209494

Original publications

• 17483215,10746760,21737930